One-dimensional (1-D) Ag arrays were formed by electrohydrodynamic jet-printing (EHD) of polyvinylpyrrolidone (PVP, 1,300 k, Aldrich) solution ink. The 1-D Ag arrays were formed on slide glass by controlling the viscosity and printing conditions such as the tip to the substrate distance, the applied voltage, the flow rate of ink and the velocity. The printed pillars were dried at 80 °C to get rid of the solvent and sintered at 400 °C for 30 min. We found that the arrays of Ag pillars can be fabricated by using the EHD printing method. We could control the diameter of the pillars in the range of 100–200 μm and the length rage between 300 and 700 μm. In order to produce a better performance of glucose sensor, we infiltrated a glucose oxidase as a glucose detector into the Ag pillars previously coated with the mixed solution of multi-walled carbon nanotube (MWCNT, Iljin), Nafion and Pt nanoparticles. In addition, the Ag array electrode with glucose oxidase was used as a working electrode for glucose detection via the three-electrode electrochemical method.